The Rebels, featuring three new signings in the starting eleven and with three more on the bench, started well, although the first chance fell to Aylesbury on six minutes when a delightful ball found Rudd, but his header went just over.
Sean Sonner almost broke the deadlock after latching on to a through ball, but he was adjudged to have been offside.
In a fast-paced opening, Sonner flashed another shot wide, and new Rebels goalkeeper Steve Smith, facing his former club, made a good save down low to his left.
With Slough playing a 3-5-2 formation with Danny Murphy and Sinclair as wing-backs, Aylesbury began to get on top and see more of the ball, exploiting Slough’s narrowness in midfield – but the Slough rearguard, marshalled by Dave Woozley, stood firm and nullified the threat of a visiting Aylesbury attack, which included former Slough player Simon Martin - one of nine debutants.
Danny Murphy’s free kick was well held by goalkeeper Ender, and that was as close as either side came to scoring in the opening 45 minutes.
After this sluggish start to the season, Slough emerged from the half-time break looking to make an impact, and Murphy nearly latched on to a loose ball when one-on-one, but just failed to reach the ball.
A game-ending injury to Aylesbury skipper Ben Stevens forced a reshuffle and, not long after, the best chance of the game fell to Sonner. The second highest goalscorer from last season failed to convert Burnell's cross six yards out, and was unlucky to see the chance snuffed out.
But at the other end, Aylesbury came to life, and Smith saved his side with a fantastic save low to his right as a long range effort took a massive deflection on its way through.
Rudd fired a cross-shot wide and Shaun Byrne had a shot cleared off the line as Slough threatened again, before Sinclair was played through by Coyne, but his weak shot was easily saved.
In the closing stages, Slough survived a strong Aylesbury appeal for a penalty when Martin received the ball in the box and had his shirt pulled - however not in the eyes of the referee.
Murphy fired a fierce shot which was heading straight into the top corner, but Ender saved the shot superbly and Slough had to be content with an unbeaten start to the season and a clean sheet.
Aylesbury Line up
Ender, Parsons, Mead, Stevens (c) (Kennedy), Prosper, Thomson, Simpson, Schmidt, Martin, Vicente (Williams), Rudd (Squire-Adams). Subs not used: Tallyn, Watson.